Christopher Nguyen1, Peter Speier2, Xiaoming Bi3, and Debiao Li1,4
1Biomedical Imaging Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA, United States, 2Siemens Healthcare GmbH, Erlangen, Germany, 3Siemens Healthcare, Los Angeles, CA, United States, 4Bioengineering, University of California Los Angeles, Los Angeles, CA, United States
Synopsis
Clinical application of
diffusion-weighted cardiac magnetic resonance (DW-CMR) has demonstrated promise
in detecting and characterizing the tissue microphysiology and/or
microstructure.
The aim of this study is to
compare a recently developed SE diffusion-prepared approach with a conventional
STE DW-CMR technique in normal volunteers on a 3T
system. M2-SE derived MD values
were significantly higher than STE derived values despite the same prescribed
b-value and the difference is not dependent on heart rate. Both DW-CMR
techniques were reproducible and motion corruption was seen to be overall less
in M2-SE.Introduction
Clinical application of
diffusion-weighted cardiac magnetic resonance (DW-CMR) has demonstrated promise
in detecting and characterizing the tissue microphysiology and/or
microstructure of myocarditis, myocardial edema, and myocardial infarction
1-3. However, as new DW-CMR techniques
4 emerge beyond the conventional DW-CMR
5, there is a clear difference in the absolute
quantification of mean diffusivities (MD) dependent on whether the DW data was
collected with spin echo (SE) or stimulated echo (STE) encoding. The aim of
this study is to compare a recently developed SE diffusion-prepared approach
with a conventional STE DW-CMR technique in normal volunteers on a widely
available clinical 3T system (MAGNETOM Skyra, Siemens Healthcare, Germany).
Methods
A recently developed second
order motion compensated (M2) SE diffusion-prepared approach (10 avg, TRg=2RR,
TEprep = 67ms, free-breathing, 2D single shot bSSFP readout: TR/TE = 2.7/1.4ms,
FA = 90°, R = 2, partial Fourier factor = 6/8) was compared with a conventional
STE DW-CMR technique (10 avg, breath-hold, 2D SS EPI readout: TR/TE = 2RR/20ms,
reduced FOV factor = 5, partial Fourier factor = 6/8) in healthy volunteers
(N=5). Prototype sequences implementing M2-SE and STE DW-CMR were both applied
in mid diastole to avoid strain contamination and reduce bulk motion during
encoding. Both DW-CMR techniques acquired 3 non-collinear diffusion directions
at a b-value = 350 s/mm
2 and a single b-value = 30 s/mm
2
image at the mid ventricular short axis slice. Total scan time for both
techniques were kept at ~5 minutes. Regional (6 AHA segments) myocardial MD
values (N=30 segments) were tested for significant differences using paired
t-test. Intra-scan reproducibility was tested interleaving M2-SE with STE measurements
and alternating the initial measurement to reduce bias. Bland-Altman plots were
generated to qualitatively test for Type 1 and 2 errors. Motion corruption defined
by > 10% myocardium exhibiting signal void within each AHA segment for each
DW measurement (N=90) was tallied and an overall percentage was reported. Data
deemed motion corrupt were removed from MD estimation. Dependency on heart rate
was also examined.
Results
Heart rate for all healthy
volunteers were stable (72±7 BPM) throughout the 5 minutes of scanning for each
measurement. MD values were significantly (p=0.02) higher in M2-SE (1.5±0.3 um
2/ms)
compared with STE (1.2±0.2 um
2/ms). Regional analysis of MD values
showed lateral wall variability to be significantly (p=0.03) higher in M2-SE
than STE (SD: 0.5 vs 0.2 um
2/ms, respectively). The mean bias in MD
between M2-SE and STE was relatively constant (0.4±0.2 um
2/ms) and
had weak correlation with heart rate (r2=0.1). Both methods
demonstrated high intra-scan reproducibility with low bias in repeated measures
of MD (M2-SE: 0.2 um
2/ms; STE: 0.1 um
2/ms). Overall,
M2-SE exhibited significantly less motion corruption than STE (7.8% vs 11%,
respectively). However, STE demonstrated significantly less motion corruption than
M2-SE (22% vs 13%, respectively) at higher heart rates (>80 BPM).
Discussion
The disparity between MD estimations between M2-SE and STE
is consistent with other groups’ findings for brain, skeletal muscle, and heart
6-8. These studies hypothesize
that the large difference in diffusion mixing times may be the source for the
disparity. Our work is supportive of this claim demonstrating the disparity in
MD estimation is not dependent on heart rate, which may have been confounding
source for in vivo DW-CMR imaging. Another confounding source may be STE’s
dependency on strain, which in this study was mitigated by “sweet spot”
triggering. A more complete future comparison study would include multiple
triggering throughout the cardiac cycle.
Conclusion
M2-SE derived MD values were significantly higher than STE
derived values despite the same prescribed b-value and the difference is not
dependent on heart rate. Both DW-CMR techniques were reproducible and motion
corruption was seen to be overall less in M2-SE. Higher stable heart rates decreased
M2-SE’s motion robustness and caused more variations in lateral wall MD values
compared with STE.
Acknowledgements
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