Characterization of carotid plaque using sampling perfection with application-optimized contrast using different flip angle evolution (SPACE) sequence
Yoshimitsu Ohgiya1, Nobuyuki Ohike 2, Naomi Yagi 1, Hiroto Sasamori1, Jiro Munechika 1, Masanori Hirose 1, and Takehiko Gokan 1

1Showa University School of Medicine, Tokyo, Japan, 2Department of Pathology, Showa University Fujigaoka Hospital, Yokohama-shi, Japan

Synopsis

The purpose of this study was to investigate whether 3D turbo spin-echo (TSE) SPACE sequence can evaluate plaque characteristics of the carotid artery. Mann-Whitney test was used to examine differences in the contrast ratio (CR) between soft plaques and fibrous plaques on T1- and T2-weighted images. The mean CR of the soft plaques (1.54 ± 0.25) was significantly higher than that of the fibrous plaques (1.17 ± 0.13) (p < 0.001) on T1-weighted images. In conclusion, T1-weighted TSE SPACE sequence can evaluate main components of plaques in the carotid artery with high sensitivity and specificity.

PURPOSE

To investigate whether three-dimensional variable-flip-angle turbo spin-echo (SPACE) sequence can evaluate plaque characteristics of the carotid artery.

METHODS

This study included 18 consecutive patients (17 men, 1 woman; mean age, 71.1 years) who underwent T1- and T2-weighted SPACE sequences and carotid endarterectomy from June 2013 to May 2014. All MRI examinations were performed using a 1.5-T MR scanner (MAGNETOM Essenza; Siemens Medical Solutions, Erlagen, Germany). T1-weighted SPACE (repetition time ms/echo time ms/ flip angle/ turbo factor = 784/13/variable/37; voxel size, 1.0 × 1.0 × 1.0 mm3; field of view, 253 mm; GRAPPA factor, 2; Acquisition time, 3 min 47 sec) and T2-weighted SPACE (1400/193/variable/95; voxel size, 1.0 × 1.0 × 1.0 mm3; field of view, 253 mm; GRAPPA factor, 2; Acquisition time, 4 min 47 sec) sequences were performed in the neck including the bilateral internal carotid arteries. Signal intensities of the plaque and adjacent muscle were measured on T1- and T2-weighted images. Contrast ratio (CR) was calculated by dividing the signal intensity of the plaque by that of the muscle. A pathologist measured areas of lipid/necrotic core, hemorrhagic components, fibrous tissue, and calcification. The plaques were classified into two types: soft plaque consisting of lipid core and/or hemorrhagic components more than 50%; and fibrous plaque consisting of fibrous tissue and/or calcification more than 50%. Mann-Whitney test was used to examine differences in the CR between soft plaques and fibrous plaques on T1- and T2-weighted images.

RESULTS

Microscopic examination of endarterectomy specimens revealed 13 soft plaques and 5 fibrous plaques. Table 1 shows the CRs of the carotid plaques to adjacent muscle on T1-weighted SPACE and T2-weighted SPACE images. The mean CR of the soft plaques (1.54 ± 0.25) was significantly higher than that of the fibrous plaques (1.17 ± 0.13) (p < 0.001) on T1-weighted images (Fig 1-3). There was no significant difference in the mean CR between the soft plaques (2.31 ± 1.07) and the fibrous plaques (2.33 ± 0.98) on T2-weighted images. When the CR cut-off value was set at 1.28, soft plaques were diagnosed with 92.3% sensitivity, 80.0% specificity, and 88.8% accuracy (Fig 4).

DISCUSSION

Plaque MR imaging is typically performed using 2D turbo spin-echo (TSE) sequence, which offers flexible tissue contrast, less sensitivity to magnetic field inhomogeneities, and high signal-to-noise ratio (SNR). A common problem in 2D imaging is the poor spatial resolution in the slice-select direction, making images more prone to the partial volume effect and obscurity of fine plaque structures. In contrast, 3D imaging has several advantages. 1) Intrinsically high SNR that may be traded for high spatial resolution. 2) Improved anatomic coverage via a longitudinal acquisition. 3) Capability of retrospective visualization of vessel wall from arbitrary angle using MPR. In our study, the main components of the carotid plaque were distinguished with high sensitivity and specificity by using SPACE sequence. This is because the T1-weighted SPACE sequence has T1 contrast and an inherent black-blood effect due to variable flip angles. In the discrimination of soft plaques from fibrous plaques, the sensitivity and specificity of T1-weighted SPACE sequence in our study were comparable with previous reports (79%-96% and 84%-100%, respectively) 1-6). The T2-weighted SPACE sequence was not useful to distinguish soft plaques from fibrous plaques, partly because TR was shorter than usual TR (e.g. 3000 msec).

CONCLUSION

T1-weighted SPACE sequence can evaluate main components of plaques in the carotid artery with high sensitivity and specificity.

Acknowledgements

No acknowledgement found.

References

1. Yoshida K, Narumi O, Chin M, et al. Characterization of Carotid Atherosclerosis and Detection of Soft Plaque with Use of Black-Blood MR Imaging. AJNR Am J Neuroradiol. 2008;29(5):868-874.

2. Watanabe Y, Nagayama M, Suga T, et al.Characterization of atherosclerotic plaque of carotid arteries with histopathological correlation: vascular wall MR imaging vs. color Doppler ultrasonography (US). J Magn Reson Imaging. 2008;28(2):478-485.

3. Cappendijk VC, Cleutjens KBJM, Kessels AGH, et al. Assessment of Human Atherosclerotic Carotid Plaque Components with Multisequence MR Imaging: Initial Experience. Radiology. 2005;234(2):487-492.

4. Narumi S, Sasaki M, Ohba H, et al. Prediction of Carotid Plaque Characteristics Using Non-Gated MR Imaging: Correlation with Endarterectomy Specimens. AJNR Am J Neuroradiol. 2013;34(1):191-197.

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6. Yuan C, Mitsumori LM, Ferguson MS, et al. In Vivo Accuracy of Multispectral Magnetic Resonance Imaging for Identifying Lipid-Rich Necrotic Cores and Intraplaque Hemorrhage in Advanced Human Carotid Plaques. Circulation. 2001;104(17):2051-2056.

Figures

Table 1. Contrast ratios of the carotid plaques to adjacent muscle on T1-weighted SPACE (T1WI) and T2-weighted SPACE images (T2WI)

Fig 1. Imaging of carotid plaque containing mainly hemorrhage (75-year-old man with left carotid stenosis). The plaque shows evident hyperintensity (arrow) on T1-weighted SPACE image (T1WI) and hypointensity (arrow) on T2-weighted SPACE image (T2WI). The plaque contains massive hemorrhage on the specimens.

Fig 2. Imaging of carotid plaque containing mainly lipid and/or necrosis (68-year-old man with right carotid stenosis). The plaque shows mild hyperintensity (arrow) on T1-weighted SPACE image (T1WI) and mild hyperintensity (arrow) on T2-weighted SPACE image (T2WI). The plaque contains necrotic tissue and lipid on the specimens.


Fig 3. Imaging of carotid plaque containing mainly fibrous tissue (63-year-old man with right carotid stenosis). The plaque shows isointensity to adjacent muscle (arrow) on T1-weighted SPACE image (T1WI) and mild hyperintensity (arrow) on T2-weighted SPACE image (T2WI). The plaque contains thick fibrous tissue on the specimens.


Fig 4. Correlation between SPACE sequences and histologic analysis of carotid plaques



Proc. Intl. Soc. Mag. Reson. Med. 24 (2016)
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