Aortic valve disease is a condition where the three-leaved valve between heart left ventricle and aorta is not working properly. It may be either not opening fully causing obstruction to blood flow (aortic valve stenosis) or it is not closing properly resulting in a leakage and backward blood flow (aortic valve regurgitation) ¹. The non-invasive cardiac MRI has been found potential in imaging aortic valve stenosis and regurgitation, but there is a room for improvement in the image resolution to be able to accurately detect the accumulation of atherosclerotic plaque and calcification in the valve ^2,3,4. In this study, high resolution cine-imaging was applied for detecting atherosclerotic plaque accumulation in the aortic valves in hyperlipidemic mice undergoing high fat diet.6 LDLR-/-ApoB100/100 mice were on a high fat diet (42 % of calories from fat) for 5 months. In addition, one C57BL/6 control mouse was included in this study for comparison. The mice were imaged at 7T Bruker Pharmascan using volume transmitter and surface receiver coil. The MRI scans consisted of 6-8 cine-images covering the whole heart from apex to aorta to examine the heart function. Parameters for cine imaging were the following; TR =8 ms, TE = 2 ms, FOV = 4 x 4 cm², matrix size 192 x 192, 1 slice, slice thickness 1 mm, 10-12 frames depending on heart rate, and 2 averages. The size of the left ventricle was determined from the end diastolic and end systolic images from each slice and combined to obtain the end diastolic and end systolic volumes (EDV and ESV, respectively. From these the ejection fraction (EF) was calculated: EF = 1-EDV/ESV. End systolic aortic valve function was examined by imaging the cross section of the aorta at valve level using cine sequence with slice thickness of 0.6 mm. Other parameters were the following; TR = 8 ms, TE = 2 ms, FOV = 1,5 x 3 cm², matrix size 192 x 192, 1 slice, and 3 averages. From these images, the area of aortic valve orifice was determined and compared to the total area of aorta.From the cine images, the aorta wall and the accumulated atherosclerotic plaque was clearly separated. The valve function was limited (Figure 1.) suggesting stenosis/regurgitation in the mice with high fat diet. The area of the orifice was 43 ± 5 % (mean ± SEM) of the total area of aorta in these mice. EF of 6 mice was 60 ± 2 % (mean ± SEM) being lower than reported values from normal mice ⁵.We were able to clearly visualize the dysfunction of the aortic valves and accumulated atherosclerotic plaque to the mouse aortic valve. The cine-MRI provides a non-invasive tool to monitor aortic atherosclerotic plaques.