Pathology is performed for diagnosis and staging of the tumor to determine adjuvant treatment strategy. In clinical practice, several small representative tissue blocks are chosen from a large specimen to investigate the tumor differentiation, tumor stage and resection margin after tissue preservation. These blocks are sliced in micrometer thick slices for histological examination. High resolution MR imaging of full organs before sectioning can provide detailed localization and extension of tumor tissue. As tumor and healthy tissue show high contrast on T2-weighted images, the MR images can be a potential guidance for the histological examination. Here, we demonstrate high resolution MR imaging of ex vivo pancreas-specimen while maintaining the in vivo shape and orientation.The pancreaticoduodenal specimen, excised via a classic Whipple procedure, was sampled for fresh tumor samples during gross pathological examination. Any incision used for tissue sampling was stitched. The pancreas was then positioned on a plastic grid according anatomical landmarks labeled by surgeons to mimic the in vivo shape and orientation. To allow for correct 3D positioning of the specimen, a plastic tube served as a substitute for the superior mesenteric vein (SMV) as the pancreas neck rests on the ventral side of the SMV whilst the pancreatic head and duodenum are positioned on the dorsal side. Remaining incisions by PA are stitched such that the pancreas regains the in vivo shape where after it is submerged in formaldehyde (4%). The specimen was positioned in a 3T MRI system (Philips Ingenia MRI, Philips Healthcare, Netherlands, Best) and imaged using the posterior coil integrated in the table in combination with two small surface coils in a matched orientation. MRI was performed using 3D T2 weighted turbo spin echo sequence (TSE) with the following acquisition parameters: TR, 3341ms; echo time, 100ms, echo train length, 22; field of view 300x300mm2; acquisition pixel size, 0.45mm; slice thickness, 2mm; number sampled averages, 2.Multiple anatomical structures, such as the Common Bile Duct (CBD), pancreatic duct, duodenal papilla and the pylorus are visible in both figures 1 and 2. The SMV substitute appears as a signal-less object and delineates the pancreatic head from the pancreatic body. In addition, a high contrast difference is seen between tumor and healthy tissue. The tumor showed a high signal intensity (SI) on the T2 weighted images while the pancreatic tissue has lower SI as is similar to the in-vivo MR imaging of the tumor. On the in-vivo CT the tumor was measured 3cm while on the MR images the tumor measured a maximum width of 4.0 cm.High resolution imaging of ex-vivo pancreas specimen showed the exact orientation and extent of the tumor in the pathological specimen, providing a full 3 dimensional anatomical understanding. In addition, the in-vivo orientation of the specimen was clearly visible, allowing for spatial localization further improving guidance during PA and registration with in vivo MRI. The high image resolution in combination with the good SNR seen in the images, holds promise for future quantification studies.[A1] [A1]LAten staan of weghalen Marielle vindt het uit de lucht vallen