Synopsis
Limb girdle muscular dystrophies (LGMD) are a heterogeneous family of
disorders characterized by the substitution of muscles with fat and fibrotic
tissue. In this work we show the initial results of our acquisition protocol,
that included DW-MRI, T2 mapping
and DIXON imaging, on two subtypes of LGMD (type
2A and 2B). Statistical tests and Pearson’s correlation were performed on
parametric maps at single muscle level. Preliminary results show that
multi-parametric MRI is promising in the characterization of LGMD subtypes on
the thigh. Considered MRI techniques show different sensibilities to damages
induced by muscular dystrophies
and can be considered complimentary.Purpose
Limb
girdle muscular dystrophies (LGMD) are a heterogeneous family of disorders characterized by the substitution
of muscles with fat and fibrotic tissue, with a predominant involvement of the
shoulder and pelvic girdle
1,2. In this work we show the initial results of our acquisition protocol
and analysis pipeline targeted to a muscle level characterization in two
subtypes of LGMD.
Methods
Four
healthy volunteers(HCs) (35±7 years old, 2M and 2F), 6 patients affected by LGMD2A
(Calpain 3 deficiency, 42±10 years old, 2M and 4F) and 2 patients affected by LGMD2B (Dysferlin deficiency, 60±15
years old, 1M and 1F) underwent MRI of the thigh with
a 3T scanner. Acquisition protocol included a diffusion weighted (DW) sequence
(TE/TR=42/7000ms, 5 b=0s/mm
2, 15 directions b=250s/mm
2,
15 directions b=400s/mm
2, 4 directions at b=50,100,150,200,300s/mm
2,
resolution 1.5x1.5x6mm
3), a multi-echo sequence (15 echoes,TE
1
9.3ms,δTE 12.5ms,1.7x1.7x5mm
3)
for T
2 quantification and a DIXON sequence (12 echoes, TE
1
2.7ms,δTE 1.2ms) for fat fraction (FF) quantification. DW data was
pre-processed with FSL
3,4, then fractional anisotropy (FA) and mean diffusivity (MD) maps were
computed with Camino
5,6. T
2 images were moved to the DIXON space and, subsequently
to the DW space using a non-linear registration
7 to account for EPI distortions. Regions of interest (ROIs) were drawn
on muscles Gracilis and Vastus Lateralis, and used to compute boxplots of FA,
FF and T
2. T-tests (two sided, p=0.05) were performed to assess
differences between muscles in HC, testing Vastus against Gracilis, and to
assess differences between matching ROI of different populations, testing HC
against patients and HC against LGMD2A. Cross correlations between the
considered metrics and correlations between metrics and age (ρ) were computed
for each ROI as attempt to disentangle the information from each sequence.
Results
LGMD2B
patients showed, on structural T2-w images, a diffuse pattern of muscle
degeneration and fatty infiltration involving both anterior and posterior thigh
muscles, while LGMD2A patients had a more heterogeneous pattern of
degeneration, involving posterior muscles more severely (Figure 1). Figure 2 shows an example slice of the
maps obtained from a HC and a patient affected by LGMD2A. FA, FF and T
2
maps are homogeneous for the muscle of the HC subject, conversely on the
patients, FA exhibits high heterogeneity, FF is increased within the muscle,
and T
2 values are higher, especially in the Vastus. Figure 3 reports
the boxplots of all parametric maps for both ROIs. FA, FF and T
2
values were consistent for the HCs, with different MD (p=0.029) and FA (p=0.045)
values between Vastus and Gracilis. Parametric maps values between HC and
patients were different in the Vastus (FA p=0.050, FF p=0.001, T
2
p=0.037) and in the Gracilis (FF p=0.012). Considering only LGMD2A patients
against HCs, significant differences were found for both the Vastus (FA,
p=0.048, FF p=0.002) and the Gracilis (FF p=0.018). Significant correlations
were found in the Gracilis between FF and both FA (ρ=0.81, p=0.015) and MD (ρ=-0.78,
p=0.024) for patients (Figure 4). Conversely, correlations were not significant
in the Vastus, as well as correlations between T
2 and FA/MD in both
muscles. Age was significantly correlated only with Gracilis FA in the LGMD2A
group, ρ=-0.85 with p=0.033, meaning that FA decreases over time for this
group. Same correlation in the HC group was not significant but opposite in
sign. No significant correlations between age and metrics were found in the
whole group of patients.
Discussion
All
considered parameters showed stable and homogeneous distributions (Figure 2) in
the HCs, while patients were characterized by variable and heterogeneous values,
even across the same dystrophy subtype, highlighting the variety of LGMD
effects. FF was significantly different in all performed t-tests, showing to be
a very sensible marker of muscle substitution process. All considered
quantities were significantly different in muscle Vastus, that it is probably
affected by the majority of considered dystrophies. The Gracilis seems to be
less (or later) affected, especially in the LGMD2A group. Correlations between
FF and the other computed metrics were high in the Gracilis of patients, nonetheless
FA was the only measure significantly correlated to age in the LGMD2A group. The
absence of correlation between age and metrics in whole patients group can be
due to heterogeneity of the group itself.
Conclusion
Preliminary results of this study are promising in the
characterization of LGMD subtypes on the thigh. Considered MRI techniques show
different sensibilities to damages induced my muscular dystrophies and can be
considered complimentary. The limited sample size requires the results to be
confirmed on a larger cohort of subjects.
Acknowledgements
No acknowledgement found.References
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