Investigation of hypoxia conditions using oxygenation enhance (OE)-MRI measurements in C6 glioma models
Yingwei Wu1, Yongming Dai2, Qi Fan1, and Xianfeng Tao1

1Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China, People's Republic of, 2Philips Healthcare, Shanghai, China, People's Republic of

Synopsis

We used oxygenation enhancement (OE)-MRI measurements to investigate hypoxia conditions of gliomas and to evaluate relationship between histopathology measurements and PSC. Oxygen amplitude maps of C6 glioma models were derived. ROI max and ROI non-max were defined. Time-SI curve from ROI areas was obtained and tissues from ROI areas was evaluated for microvessel density and expression of HIF-1a. We found that microvessel density in ROI non-max area were lower than those in ROI max area and expression of HIF-1α in ROI non-max area were higher than that in ROI max area. PSC had a linear positive correlation with vessel density.

Purpose

To investigate hypoxia conditions of brain glioma using oxygenation enhance (OE)-MRI measurements and to evaluate correlation between histopathology measurement of tumor vascularity , expression of hypoxia-regulated molecules and percentage of signal intensity changes (PSC) before and after oxygen administration .

Methods

10^6 C6 glioma cells were implanted in rat brains using standard protocals (n=5). OE-MRI using T1-weighted inversion recovery (IR) turbo spin echo (TSE) was performed on a 3.0-T MR scanner, including dynamic T1-weighted IR TSE imaging covering 1) normal room air administration (21% O2) for 5 min; 2) 100% oxygen administration for 5 min; and 3) normal room air administration (21% O2) for 5 min continuously. Oxygen amplitude map were derived from the T1-weighted OE-MRI images, of which each voxel represents the highest percentage of signal intensity changes (PSC) before and after oxygen administration. Regions of interest (ROIs), each contains 4 voxels, are defined as ROI max (PSC > 15%) and ROI non-max (5% < PSC < 10%) on the oxygenation amplitude map. Time-SI (signal intensity) Curve for both ROI max (PSC > 15%) and ROI non-max (5% < PSC < 10%) was obtained using IR-TSE sequence. Tissue obtained from areas of ROI max and ROI non-max was evaluated for microvessel density and expression of hypoxia-inducible factor-1 a (HIF-1a). Spearman rank correlation coefficient was used to identify correlation between PSC and microvessel density. Paired Student tests were used to compare the PSC, microvessel density, and HIF-1a expression from ROI max with the ROI non-max.

Results

Results: PSC of OE-MRI signal regionally varied in C6 glioma models. Both PSC and microvessel density in ROI non-max area were significantly lower than those in ROI max area (P < 0.05). Consistent with MRI results, expressions of HIF-1α in ROI non-max area were obviously higher than that in ROI max area(P < 0.05). Furthermore, PSC had a linear positive correlation with vessel density (r=0.92, P < 0.05).

conclusion

OE-MRI measurements were capable to demonstrate hypoxia conditions in C6 glioma models. PSC was a valuable parameter for evaluation of tumor vascularity and expression of hypoxia-regulated molecules

Acknowledgements

No acknowledgement found.

References

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Figures

Oxygen amplitude map of a C6 glioma model.

Time-SI (signal intensity) curve from ROI max (PSC > 15%) and ROI non-max (5% < PSC < 10%) areas

Expression of CD31 in ROI max area.Arrows indicate blood vessels,

Expression of CD31 in ROI nonmax area.Arrows indicate blood vessels,

Immunoblotanalysis using tissue from ROI max area and ROI nonmax area



Proc. Intl. Soc. Mag. Reson. Med. 24 (2016)
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