Ultra-short TE (UTE) R2* mapping and Quantitative Susceptibility Mapping (QSM) are emerging techniques for quantifying iron deposition in various organs, including the brain and liver. In tissues with short T2* values (high R2*), the fast signal decay-induced errors during the relatively long readout in typical UTE acquisitions, i.e., 3D radial and cones UTE, may confound R2* and susceptibility measurements. In this study, we characterized the readout duration effects on R2* and susceptibility estimation in 3D radial and cones UTE-acquisitions at 3.0T. Simulation and phantom studies showed bias in the estimated R2* and susceptibility when long readout durations were used.
Simulation: A numerical phantom was created containing five vials with different R2* (550~1979s-1) and susceptibility values (1-5ppm), according to the reported values of iron-overloaded livers5. 3D images at six echo times with center-out radial and cones trajectories were simulated following the gradient-echo signal model6 including R2* signal decay and susceptibility related phase accumulation during readout duration. For each dataset of the multi-echo images, four readout durations of 0, 1, 2 and 3ms were used. Detailed phantom MRI properties are listed in Table 1.
Phantom Study: The phantom consists of six vials placed in a MnCl2-doped water bath, including five vials of mixed CuSO4-MnCl2 solutions to obtain a range of R2* and susceptibility values similar to the reported ones in iron-overloaded livers5, and one deionized water vial as a susceptibility reference. Phantoms were imaged on a 3.0T scanner (Discovery MR750, GE Healthcare, Waukesha, WI). 3D center-out radial acquisitions with five different readout durations (3.22~0.59ms) were obtained by adjusting the readout bandwidth (15.63~125kHz, respectively). Six echoes (echo times=0.5~3.0ms) were acquired with each readout duration for R2* mapping and QSM. Phantom details and other MRI scan parameters are listed in Table 1.
Data Reconstruction and Analysis: R2* maps and field maps were reconstructed from each multi-echo dataset in simulation and phantom studies using a complex-fitting algorithm7. Background field removal was achieved by subtracting the field map of all vials assigned with water susceptibilities in simulation and using projection onto dipole fields (PDF)8 in phantom studies. Susceptibility maps were calculated using morphology enabled dipole inversion (MEDI)9-11. R2* and susceptibility were measured in a cylindrical region of interest (ROI) drawn in each vial, and the deionized water vial was used as the susceptibility reference. A boundary B0 field measurement ∆B0, which is directly proportional to susceptibility difference between two regions12, was measured as the field difference between ROIs inside and outside each vial near the boundary parallel to the main magnetic field.
Our simulation and phantom studies consistently demonstrate that long readout duration leads to bias in R2* and susceptibility measurements in 3D radial and cones UTE-acquisitions, especially at high R2* and susceptibility values at 3.0T. Signal decay and phase accumulation during the readout introduce errors in R2* and field map estimations, and these errors propagate through the susceptibility reconstruction into final estimates of susceptibility. These findings may serve as a guide for the development of R2* mapping and QSM techniques based on non-Cartesian acquisitions, which are particularly promising in abdominal imaging applications due to their ability to provide short echo times and high motion robustness. This study had several limitations, including the lack of 3D cones acquisition in phantom study and in vivo study, and the potential geometry dependence of the observed bias. Additional phantom and in vivo studies are needed to fully characterize this effect.
In summary, this preliminary characterization provides insight into the bias in R2* mapping and QSM performed using non-Cartesian acquisitions.
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