The “traveling heads” is a study to assess the comparability and reproducibility of multicenter human brain imaging at 7T. In previous experiments, we compared typical UHF sequences for structural brain imaging. In this study, we focus on the reproducibility of quantitative imaging and compare methods for volumetry, relaxometry, QSM and CEST between different sites. In addition, three generations of 7T MR systems are compared, i.e. the older installed base consisting of passively and actively shielded magnets of the first and second generation, respectively, as well as the most recent generation which has been approved as a medical device.
Methods
Two male subjects (40 and 36 yrs.) were imaged at three UHF sites, all operating a 7T whole-body MR system from the same vendor (Siemens Healthcare GmbH, Germany). The systems represent three different generations of 7T with two different software platforms and differences in basic imaging components that might influence image quality. The oldest device is a passively shielded magnet (Agilent, UK) equipped with a 38 mT/m gradient coil (PS site). Site 2 uses an actively shielded Agilent magnet of the second generation with a 70 mT/m gradient coil (AS site). Both sites run the same (discontinued) software platform (VB17). At Site 3 (Terra site) the newest (CE-labeled) generation of an actively shielded, compact magnet and an 80 mT/m gradient coil as well as a different software platform (VE11) are installed. All sites are equipped with a commercially available RF head coil (Nova Medical) with 1 TX and 32 RX channels that was used for the measurements.
The imaging protocol started with a calibration part (Fig. 1) consisting of DREAM(1) B1 mapping (TA: 0:05 min, 5 mm isotropic) for transmitter calibration followed by a GRE B0 field map (TA: 2:03, 3 mm isotropic) to measure the reproducibility of the adjustment routines.
A MP2RAGE (TA: 9:38 min, 0.75 mm isotropic) sequence was used for volumetric measurement, and FSL(2) was used for image processing / registration (Fig. 2) and to compare the gathered data with previous traveling heads experiments(3).
For QSM (Fig. 3) a multi-echo (ME) GRE sequence (TA: 10:11 min, 5 echoes, 0.7 mm isotropic) was acquired that uses ASPIRE(4) for phase combination of the acquisition. The phase maps of each echo time were processed with a Laplacian-based phase unwrapping algorithm(5,6) to remove phase wraps and V-SHARP to eliminate background fields(7). Finally, the susceptibility map was obtained by using STAR-QSM(8). An echo-time-averaged susceptibility map was created by using the squared echo time and the squared signal magnitude as a weight(9).
CEST MRI (Fig. 4) was realized based on a previously optimized acquisition protocol for 7T(10). The protocol comprises a low-power pre-saturation, a 5-pool Lorentzian-fit analysis, and a correction for B0 and B1 inhomogeneities. However, in contrast to the previous protocol, the image readout was extended to three dimensions (1.7×1.7×3 mm3, 18 slices, TA: 16 min) using the snapshot-CEST approach(11) with adapted imaging parameters. In addition, Z-spectra were de-noised using a principal component analysis. The final CEST contrasts (APT and rNOE) were calculated by the inverse metric(12).
Quantitative maps of R1, R2* and PD (Fig. 5) were acquired using a multi-parametric mapping technique(13) further adapted for 7T and for increased isotropic resolution. The protocol consisted of two 3D ME GRE scans with T1 and proton density (PD) weightings (TA: 2x 9:51 min, FA: 21/5°, 6 echoes, 0.65 mm isotropic). Maps were calculated with the hMRI toolbox (http://hmri.info) within the SPM 12 framework.
The total measurement time was 60 min per subject.
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