Hyperpolarized (HP) media enable biomedical imaging applications otherwise unachievable using standard MRI contrast agents. However, quantitative analysis of HP images is complicated by spatially varying signal decay due to T1 relaxation and RF excitation. By imaging with center-out trajectories, HP signal intensity is collected with every acquisition, thereby encoding signal decay alongside k-space data. Using keyhole reconstruction, multiple temporally resolved images were reconstructed to generate voxel-by-voxel maps of hyperpolarized signal decay following a simple analytical model. Here these maps are applied to correct spatially varying magnetization decay in HP 129Xe, improving the quantitative accuracy of ventilation images.
Hyperpolarized (HP) media MRI, particularly gaseous HP 129Xe imaging, is increasingly used for pulmonary structural and functional imaging of conventionally hard-to-image anatomy and physiological processes.1-4 However, the quantitative accuracy of these images is deleteriously impacted by spatially varying polarization decay due to longitudinal relaxation and RF pulsing.5-6 This is especially problematic for human lung imaging, where large coils lead to significant B1 variability and heterogeneous O2 content leads to significant T1 variability. To alleviate these concerns, T1 and flip angle (B1) maps can be generated, but this mapping consumes additional hyperpolarized magnetization, increasing the time and expense of scans.7 In principle, however, both T1 and RF depletion are encoded simultaneously with imaging, suggesting that HP magnetization decay can be extracted from imaging data given appropriate selection of imaging sequences and reconstruction algorithms.
Imaging sequences with center-out k-space trajectories (3D spiral, radial, etc.) sample the total HP signal intensity (k0) with each view, thus encoding signal decay within the time-domain data.8-10 Because structural detail remains constant during a short (<16s) scan, temporal subsets of low-frequency k-space may be combined with the entirety of the high-frequency edges to create time-resolved images from a single dataset, i.e. keyhole reconstruction.11-12 Here we demonstrate that by combining HP media images obtained using center-out k-space trajectories with post-acquisition keyhole reconstruction, voxel-by-voxel HP signal decay maps can be generated with no additional data collection.
Validation studies: Radial sampled k-space data were simulated to mimic spatially varying signal decay. Additionally, both structured and non-structured phantoms containing hyperpolarized 129Xe were imaged using 3D radial ultra-short echo time (UTE) sequences on both clinical (3T Achieva, Philips Healthcare, Best, Netherlands) and preclinical (7T BioSpec, Bruker, Billerica, MA) imaging systems. Clinical(Preclinical) scanning parameters: matrix: 44×44×44(40×40×40), FOV: 250×250×250(40×40×40)mm3, TR/TE: 4/0.14(6/0.7)ms, bandwidth: 14,000(50,000)Hz, views: 1940(2514), α: 0.5-3°(1-3.5°)
In Vivo studies: In vivo 129Xe ventilation images were obtained using 3D radial imaging on a 3T Achieva system for 6 subjects (N=4 healthy, N=2 with cystic fibrosis, mean age 15.8±7.9yrs, 3 male, 3 female). Parameters included: matrix: 60x60x60, FOV: 300x300x300mm3, TR/TE: 4.5/0.15ms, bandwidth: 265.0Hz/voxel, views: 3600, α: 1-1.5°). For all imaging, xenon was polarized to ~25% (Polarean model 9810, Durham, NC).
Keyhole: Low frequency k-space data determined by the Nyquist radius was separated into two temporally divided (1st/2nd half of acquisition) datasets and reconstructed with all of the high frequency data (Figure 1) using in-house reconstruction software or graphical programming interface (GPI).13-15 These “keyhole” images were used to generate maps of regional hyperpolarized signal decay (C1=cos(α)exp(-TR/T1)) following a simple analytical model. Maps of regional decay were then used to correct images reconstructed from the complete k-space data.
For simulations, phantom imaging, and in vivo imaging, mean decay values closely matched global measures of decay, and regional variations matched those expected from experimental parameters and lung physiology. Bland-Altman analysis from phantoms with negligible T1 further demonstrate close agreement between extracted and global decay. Finally, decay maps were used to correct ventilation images for regional hyperpolarized signal decay. After correction, there was minimal change in healthy ventilation images, but the ventilation pattern displayed by CF subjects is significantly altered (Figure 4), emphasizing the role magnetization dynamics play in attempts to quantitatively image HP media.
By sampling k-space with center-out k-space trajectories and using post-acquisition keyhole reconstruction strategies, hyperpolarization decay maps may be generated using a single image dataset without additional data collection. While this technique was validated and applied using hyperpolarized 129Xe MRI, the approach is general and can be applied to the imaging of any type of HP media.
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