The cardiac and respiratory response functions derived from RETROICOR have substantial variation. We found the cardiac response function is phase, or time shifted across the brain while the respiratory function has a fixed phase, but changes polarity across the brain. Based on this finding, we propose a cardiac and respiratory noise correction method in rsfMRI data without external cardiac and respiratory fluctuation measures, and compare its performance to the RETROICOR model.
Twenty eight healthy controls were scanned at 3T using single band EPI with pulse plethysmograph and respiratory belt recording (TR=2.8s, 128x128 matrix, 31 slices, 132 repetitions). After the first 4 volumes were removed, the second harmonic RETROICOR was applied using modified RetroTS.m (https://afni.nimh.nih.gov/sscc/staff/glend/matlab_compiler). RETROICOR includes the physiologic noise removal process to minimize the error of physiologic regressor model fit. In this study, “RETROICOR model” indicates Fourier series of regressor fitting based on the defined phase. We calculated the voxelwise cardiac and respiratory HRFs from the top 5% of F value voxels in RETROICOR model for the development and validation of the proposed cPESTICA.
PESTICA generates the physiologic noise estimator using slicewise ICA (3). The first, we define the highest peaks from the estimated cardiac noise estimators from PESTICA, as shown in Fig3, and the linear phase in the time domain from 0 to 2π between the peaks. Note that this phase, defined from PESTICA is not phase from the actual cardiac pulsation measures. The second order Fourier Series were modeled over the defined phase in the same ways that RETROICOR model doesTherefore, cPESTICA has 5 degree of freedom (DOF) while the original PESTICA and RETROICOR have 2 and 8 DOFs.
While HRF is NOT used for cPESTICA or RETROICOR correction, HRFs over phase between cPESTICA and RETROICOR are compared for the validation purpose. F value and t-score maps from cPESTICA and RETROICOR are presented.
We found that cPESTICA estimates cardiac noise regressors that are equivalent to those produced from the RETROICOR model. Our observed high correlation of the cardiac HRF between cPESTICA and RETROICOR model indicates that cPESTICA can provide reliable physiologic estimators when external physiologic noise measures are not available during fMRI scanning or when the physiologic data is corrupted due to bad contacts or acquisition issues.
This study shows that physiological noise correction with single regressor is only valid for respiratory noise but limited for the time delayed cardiac noise. cPESTICA considers the shift of the cardiac HRF and removes the cardiac noise effectively.
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