Maggie Eppelheimer1, Soroush Heidari Pahlavian2, Rouzbeh Amini1, Francis Loth1, Daniel Barrow3, and John Oshinski4
1Conquer Chiari Research Center, Department of Biomedical Engineering, The University of Akron, Akron, OH, United States, 2Laboratory of FMRI Technology (LOFT), USC Stevens Neuroimaging and Informatics Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, United States, 3Department of Neurosurgery, Emory University, Atlanta, GA, United States, 4Department of Radiology & Imaging Sciences and Biomedical Engineering, Emory University, Atlanta, GA, United States
Synopsis
Chiari type I malformation (CMI) patients have altered brain morphology
and tissue motion compared to healthy controls.
This motion may be the underlying cause of Chiari symptoms, such as
occipital headaches. Highly symptomatic patients may be treated with posterior
fossa decompression (PFD) surgery to restore cerebrospinal fluid flow and
relieve brainstem compression. To quantitatively assess changes in brain tissue
motion before and after surgery, we examined brainstem and cerebellar tissue
displacement before and after surgery in 10 patients using spiral cine DENSE MRI.
We found that tissue displacement in these structures in CMI patients is
significantly reduced by PFD surgery.
Introduction
Patients with Chiari malformation type I (CMI) have altered morphology
and brain tissue motion compared to healthy individuals (1-3).
These motion alterations may be the underlying cause of symptoms due to repeated
compression of the cerebellum, brainstem, and upper cervical cord; however, this
remains unproven. Highly symptomatic CMI patients are often treated surgically with
posterior fossa decompression (PFD) surgery. The goal of this surgery is to restore
cerebrospinal fluid flow and relieve brainstem compression by altering cranio-cervical
morphology (4).
While most CMI patients have reported improvement in symptoms following PFD
surgery, improvement is not consistent across all symptoms or all subjects, and
selection of candidates for surgery is currently based on subjective
symptomology (5). Thus,
selection of surgical candidates may benefit from the ability to quantitatively
assess brain tissue motion. Brain tissue motion before and after PFD
surgery has been previously quantified at a select number of landmarks within
the cerebellum and brainstem (3).
However, tissue displacement over the entirety of these structures has not been
assessed. We have previously used displacement
encoding with stimulated echoes (DENSE) MRI to quantify sub-pixel levels of
brain tissue displacement in healthy subjects (6).
In the current study, we acquired DENSE images to determine tissue displacement
before and after PFD surgery in CMI patients. We hypothesized that motion within the cerebellum and brainstem will
significantly decrease following PFD surgery. Methods
Ten CMI participants (9
females, age 35 ± 6)
were imaged before and after PFD surgery in an IRB-approved study. CMI
diagnosis was determined through presence of a greater than 5 mm tonsillar
descent below the foramen magnum using a previous clinically-based imaging
study in addition to the presence of clinical symptoms. All patients had PFD
surgery and were referred by a single neurosurgeon (DB). The study protocol
included a cardiac-gated, spiral cine DENSE sequence performed at the
mid-sagittal plane on a 3T MRI scanner (Siemens Prisma, Erlangen, Germany).
Acquisition parameters included: two directions of in-plane motion encoding,
encoding frequency of 0.6 cycles/mm, two spiral interleaves per heartbeat,
pixel size of 1.2 × 1.2 mm2, slice thickness of 7 mm, four signal averages,
16-27 frames over the cardiac cycle, depending on the heart rate. A MATLAB (Mathworks,
Natick, MA) based program (DENSEpro) was
developed to evaluate tissue motion from the DENSE phase images. Using DENSEpro,
two regions (brainstem and cerebellum, Fig. 1) were manually segmented on
midsagittal DENSE magnitude images before and after surgery in all subjects by
a single observer (ME). The pixel-by-pixel displacement magnitudes over the
cardiac cycle were used to determine maximal tissue displacement, which was
calculated for each pixel and displayed as a displacement colormap. These maximal tissue displacement values were
then spatially averaged for the brainstem and cerebellum in each subject
(referred to as mean maximum displacement). Differences in spatially averaged maximal tissue motion before and after
surgery were determined using paired sample t-tests, and statistical
significant was evaluated as p <
0.05.
Results
All 10 subjects
completed the pre- and post-surgery imaging protocol, and the displacement maps
and spatially-averaged values of maximal displacement were determined. An
example of pre- and post-surgery displacement map for one subject is shown in
Figure 2 and illustrates the substantial reduction of displacement in the
inferior cerebellum and brainstem. Spatially-averaged (mean) values of maximum tissue
displacement in the cerebellum decreased 40% following PFD surgery (mean ±
SD: -59 ± 39
μm, p =
0.001, Figure 3). Maximum displacement within the brainstem was
reduced by 28% following PFD surgery (-60 ± 62
μm, p =
0.01, Figure 3). This reduction primarily occurred in the craniocaudal direction.Discussion
The major finding of
this study is that spiral cine DENSE imaging was able to show that PFD surgery
results in a significant reduction in cardiac induced displacement of the
cerebellum and brainstem of patients with CMI.
For the first time, quantitative
measurements of brain parenchyma displacement on a pixel by pixel basis that is
affected by PFD surgery were obtained in this study. This finding is noteworthy
as this sub-occipital region is often cited by Chiari patients as a region of
pain that often resolves with surgery. Further
studies will examine temporal characteristics of motion, motion in other brain
regions, and calculation of tissue strain.Conclusion
We have shown that tissue
displacement in the cerebellum and brainstem is significantly reduced by PFD
surgery. The use of cardiac-gated spiral cine DENSE imaging enables sub-pixel
measurements of displacement.Acknowledgements
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