We developed a realtime strain encoding technique using EPI readout with centric reordering and parallel imaging acceleration. This technique was tested in a strain phantom and normal volunteers to measure through-plane left ventricular strain. Quantified strains correlated well with fully sampled k-space acquisitions. The proposed technique allows larger field-of-view (FOV) and provides superior coverage of both left (LV) and right ventricles (RV) while maintaining equivalent temporal and spatial resolution as the fully sampled sequence.
A prototype real-time SENC pulse sequence was implemented on a 1.5T clinical scanner using EPI readout with centric reordering (MAGNETOM Avanto, Siemens Healthineers, Germany). Two echo trains each with echo-train-length 17 (total 34 acquired k-lines) were used to acquire each frame (Figure 1). GRAPPA acceleration with 16 integrated reference lines was combined with EPI readout to accelerate data acquisition. In each echo train, the first 8 lines in central k-space are fully sampled for kernel estimation; the remaining 9 outer k-lines are 2x under-sampled. As a result, 34 acquired lines are used to reconstruct 52 lines, for an effective acceleration rate of ~1.6. For comparison, fully sampled SENC images were also acquired with common scanning parameters including: temporal resolution 29 ms, voxel size 4.7x4.7x12 mm, FA 120, fat saturation, EPI factor 17, two shots, and receiver bandwidth 1929 Hz/pix. GRAPPA acceleration was used to increase the imaging matrix (96x52) and PE FOV (450x243 mm2) by ~1.6 times compared to fully sampled SENC acquisition (imaging matrix 96x34 and FOV 450x160 mm2) without sacrificing spatial or temporal resolution. The FOV was positioned for full coverage of the LV in both scans for comparison purposes.
A silicone strain phantom compressed by a pneumatic pump was used to assess the GRAPPA accelerated EPI SENC technique. Additionally, five healthy volunteers were scanned in six cardiac views (2CH, 3CH, and 4CH long axis views, and apical, mid-ventricular, and basal short axis). Images with fully sampled centric EPI trajectory were collected at the same location for comparison. All images were analyzed using MyoStrain 5.0 (MyoCardial Solutions, NC, USA) software to calculate global and segmental peak strain values.
GRAPPA accelerated and fully sampled EPI SENC measured peak strains of -19.7% and -21.4% respectively in the phantom with known strain of -20.0%. Figure 2 shows the need for expanding FOV in a 4-chamber view to cover both the right and left ventricles. GLS and GCS measured in 5 volunteers with GRAPPA SENC are shown in Figure 3 and compared with fully sampled SENC. Linear regression and Bland-Altman plots of segmental strains (16 longitudinal segments and 21 circumferential segments in each volunteer) are shown in Figures 4 and 5.
Across the five volunteers, global longitudinal (GLS) and global circumferential strains (GCS) demonstrated reasonable agreement between the two techniques without significant bias. Maximum absolute strain difference was 1.0%; mean GLS difference was 0.2% and mean GCS difference 0.2%. Segmental strain showed good correlation as well, with regression coefficients of 1.07 for longitudinal and 0.97 for circumferential strain, respectively. Although the mean difference was near zero (0.02% and 0.24%), segmental strains showed greater variation than the global strains, with 1.96 SD being at 5.0% and -5.1% circumferentially, and 3.0% and -2.5% longitudinally.
1. Osman, Nael F., et al. "Imaging longitudinal cardiac strain on short‐axis images using strain‐encoded MRI." Magnetic Resonance in Medicine: An Official Journal of the International Society for Magnetic Resonance in Medicine 46.2 (2001): 324-334.
2. Pan, Li, et al. "Real‐time imaging of regional myocardial function using fast‐SENC." Magnetic Resonance in Medicine: An Official Journal of the International Society for Magnetic Resonance in Medicine 55.2 (2006): 386-395.