We were able to estimate diffusion and relaxation parameters of the renal parenchyma, flowing blood and tubular water by intravoxel incoherent motion (IVIM) analysis with a three-compartment model and SE+FLAIR diffusion imaging. The reactive change of three parameters (apparent diffusion coefficient of parenchyma, transverse relaxation rate of blood, and fraction of flowing blood in the renal cortex) were observed.
The study was approved by the institutional review board and written informed consent was obtained from all study subjects. Ten healthy volunteers (mean age, 34.8 years, range, 23-67 years, men, 6) were enrolled in this study. All subjects underwent MRI examinations before and 20-40 minutes after hydration challenge (drinking water: amount of water, 20ml/kg * body weight). A 3T MR scanner (Achieva dStream, Philips Medical Systems) with 32ch ds-Torso and 32ch Posterior coils was used for the examinations. Spin-echo (SE)-based DWI and fluid-attenuated inversion- recovery (FLAIR)-DWI with short and long TE were imaged in coronal plane under free breathing. Basic imaging parameters were: slice thickness = 8 [mm], number of slice = 1, acquisition matrix = 65x112 , FOV = 230 [mm], number of signal averaged=15, b=0, 250, 500 [s mm-2]. Imaging parameters of FLAIR-DWI were: TR/TEshort/TElong = 5500/59/84 [ms] and TI =2000[ms]. Imaging parameters for SE-based DWI were: TR/TEshort/TElong = 3000/59/84 [ms].
The following parameters were estimated based on fitting three-compartment model to the 12 image data with least squares method: apparent diffusion coefficients (Dt), longitudinal relaxation rate (R1t = 1/T1t), transverse relaxation rate (R2t = 1/T2t) of renal parenchymal component, fraction (fb) and transverse relaxation rate (R2b = 1/T2b) of flowing blood component, and fraction of renal tubular water (fw). We hypothesized the following parameters with published references:2 apparent diffusion coefficients (Db,) and longitudinal relaxation time (R1b = 1/T1b) of flowing blood, apparent diffusion coefficients (Dw), longitudinal (R1w = 1/T1w) and transverse (R2w = 1/T2w) relaxation rate of water in renal tubules. The MRI signal from each component was assumed to decay exponentially by diffusion and relaxation: Exp[-D b] Exp[- R TE]; then superposed. Pared T-test was performed to evaluate changes of these parameters before and after hydration challenge. P < 0.05 was considered statistically significant.
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2. Bojorquez J, Bricq S, Acquitter C, et al. What are normal relaxation times of tissues at 3 T? Magnetic Resonance Imaging 2017;35:69–80.