In this study, we proposed a novel simultaneous multi-slice (SMS) density weighted (DW) concentric ring trajectory (CRT) metabolite-cycling Magnetic Resonance Spectroscopy Imaging (MRSI) sequence to alleviate some conventional MRSI drawbacks, e.g. long acquisition time, eddy current artifacts, and side lobe artifacts. The sequence was tested on 5 healthy subjects, showing the feasibility of acquiring three slices of high-quality water-only and metabolite spectra simultaneously with a resolution of 5mm X 5mm X 10mm within 20 minutes.
Introduction
Magnetic Resonance Spectroscopy Imaging (MRSI) allows neurochemical profiles to be acquired from multiple voxels simultaneously over substantial regions of the brain. However, this technique has been hampered by several factors, including relatively long acquisition time, side lobe artefacts, eddy-current-induced artefacts, B0 drifts due to subject motion or thermal fluctuation, and lipid contamination. In this work, we demonstrated a novel method that alleviates these issues by acquiring simultaneous multi-slice (SMS) MRSI using metabolite-cycling density-weighted (DW) concentric ring trajectory(CRT)1.Methods
Phantom and in-vivo scans were acquired using a Siemens Prisma 3-Tesla (Siemens, Germany) scanner and a 20-channel head array receive coil. Five healthy volunteers (2 males/3 females, aged 22.6 1.67 year) participated. The metabolite-cycling MRSI acquisition was achieved by the inversion of the upfield and downfield spectral ranges before the STEAM localization with a gap of 9.6 ms so that water-only and metabolite spectra can be acquired simultaneously2,3. Time-shifted multi-slice excitation RF pulse4 was simply used in place of last single-slice (z-direction) pulse of STEAM localization (TR = 1 s, TE = 16 ms) to excite three 110 mm x 80 mm x 10 mm slices (FOV= 240 mm x 240 mm) with an inter-slice distance of 20 mm. For DW-CRT, three spatial interleaves (96 unique rings) resulted in an acquisition duration of 192 s with a resolution of 5 x 5 x 10 mm3. 160 k-space sampling points were evenly distributed on each ring so that azimuthal Nyquist sampling criteria is met. Hanning‐window density weighted acquisition was used to reduce the side lobe artefact, which determines the 96 different radii of three sets of x-y rings. A blipped z-gradient scheme for each spatial interleave (0, 1, -1) was created so that an adequate FOV and resolution in the z-direction were obtained5. The number of averages was 6, corresponding to a total acquisition duration of 19.2 minutes. Corresponding single-slice data were acquired with the same parameters for the inversion of the upfield (96 s for each slice) only. SENSE method was used for reconstructions of SMS-DW-CRT data6. HLSVD is used for lipid and residual water removal7.Results and Discussion
Figure 2 shows the image derived from the SENSE reconstructed SMS and non-SMS MRSI data overlaid on the conventional MPRAGE images. The non-water-suppressed metabolite-cycling MRSI generated water images with structural information similar to that of MPRAGE for all 5 subjects. Ventricles, basal ganglia, and longitudinal fissure can be clearly distinguished. The metabolite spectra extracted from the SMS DW-CRT MRSI data are shown in Figure 3. The input spectra (blue) for LCModel have gone through eddy current correction, phase correction, zero filling, apodization, water and lipid removal, and baseline correction. The LCModel fitting results (red) indicate overall good quality of spectra for all three slices. The resulting metabolite concentration maps are displayed in Figure 4. All the voxels with tNAA or tCr CRLB higher than 50 have been discarded. Low levels of tNAA, tCr, and Glx signals (slice z=-20mm) near ventricles and elevation of Glx in the longitudinal fissure grey matter region are observed as expected (slice z=0mm). CRLB maps are displayed in Figure 5. tNAA and tCr have low CRLB values except for the highly inhomogeneous regions nearby the skull, nasal cavity, and ventricles. Glx and tCho have higher CRLB, especially in slice z=-20mm. Nevertheless, the CRLBs are relatively low in the central region of slice z=0mm and z=+20mm.
In conclusion, it is shown that the SMS DW-CRT MRSI acquisition in combination with metabolite-cycled STEAM pulse localization allows fast, robust, and high-resolution 3D MRSI at 3T.
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