Recent advances have enabled fully self-gated high resolution imaging using a Free-running framework, where data is continuously collected, retrospectively binned into cardiac and respiratory phases, and reconstructed using multi-dimensional compressed sensing (CS) for efficient functional and anatomical imaging of the heart. Here, we propose a novel expansion of this framework to cardiac and respiratory motion-resolved 3D flow imaging— or 5D flow MRI. The findings of this study show that 5D flow MRI is feasible in-vitro and in-vivo and can depict cardiac and respiratory-resolved 3D hemodynamics.
5D flow: As shown in Figure 1, a 5D flow sequence was implemented with bipolar velocity-encoding gradients in three orthogonal directions (x,y,z). 3D radial sampling following a spiral phyllotaxis pattern6 was interspersed with a self-navigation superior-inferior (SI) projections acquired at the beginning of each interleave.1 Cardiac and respiratory signals were extracted from SI profiles5 and used to bin the continuously acquired radial k-space lines into a 6D dataset (kx-ky-kz-cardiac-respiratory-flow) for reconstruction using CS. All imaging was performed at 1.5T (MAGNETOM Aera, Siemens Healthcare, Erlangen, Germany).
In-vitro: The 5D flow sequence was evaluated in-vitro using an MRI-compatible pulsatile flow pump and a U-shaped pipe. Data were acquired with isotropic spatial resolution [SR]=2.3 mm3, TE/TR/FA=3.1 ms/5.5 ms/15°, 130320 radial views, BW=600 Hz/pixel. k-space lines were sorted into 20 cardiac time frames for 43.0 ms reconstructed temporal resolution using acquired ECG timestamps, and reconstructed using a non-uniform FFT (NUFFT) or CS reconstruction. As a reference standard, a conventional Cartesian 4D flow scan was acquired with matched reconstructed SR (GRAPPA R=2, retrospective ECG-gating, acquired SR=2.3x2.3x2.8mm3, TE/TR/FA=2.36/5.1/15°, BW=455 Hz/pixel, temp res=40.8 ms).
In-vivo: 5D flow imaging was performed in 3 healthy volunteers (2M/1F, age=30-75 years, Venc=150 cm/s, TE/TR/FA=3.13-3.41ms/4.9ms/5.4ms/7°, SR=2.3-2.75 mm3, BW=450-600 Hz/pixel, 95448-130320 radial views, temp. res=49.8-51.6 ms, non-contrast) and 2 patients (post Gd-contrast, Dotarem, 2M, age=30, 38 years, BW=600 Hz/pixel, 101040 radial views, FA=15°) with bicuspid aortic valve disease. Volunteers received an additional conventional cartesian 4D flow scan of the thoracic aorta with similar reconstructed SR (retrospective gating, Venc=150 cm/s, acquired SR=2.4-2.75x3.0-3.7x3.1-4.0 mm3, temp. res.=40.4-40.8 ms, BW=455 Hz/pixel). One patient had conventional 4D flow imaging (prospectively triggered, Venc=350 cm/s, temp res=36.8 ms) as standard-of-care 27 days prior to 5D flow imaging.
In-vitro results show good-excellent visual depiction of hemodynamics (MIPS, streamlines, Fig. 2A). Time-resolved flow curves demonstrate improved agreement in CS reconstructed data versus NUFFT radial data (Fig. 2C) with conventional 4D flow, and excellent agreement of peak velocity values. Net flow values of 5D flow data were within 15% of conventional 4D flow values except in planes 4+6, which were among those further from isocenter.
In-vivo: Cardiac and respiratory signals were successfully extracted for all volunteers and patients (Fig 3.I, top). 5D flow scan times for all subjects were 8-12 minutes (8:15, 11:27, or 8:17). Representative volunteer results with differing image quality are depicted in Figure 3. 5D flow tended to underestimate peak velocities and peak flows, but showed similar systolic hemodynamic patterns and time-resolved flow curve shapes. Contrast-enhanced patient data demonstrated improved streamline visualization compared to volunteers (Fig. 4). Respiratory-resolved (4 phases) evaluation of caval venous flow from 5D flow data established the potential of the technique to quantify respiration-driven changes in net and peak flow, especially in the inferior vena cava (Fig. 5).
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